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| Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L.. |
| Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003 |
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| Alfonso-Torres,K.A.; Gargaglioni,L.H.; Pizauro,J.M.; Faria Filho,D.E.; Furlan,R.L.; Macari,M.. |
| The effect of breeder age on long bone development was studied in chicken embryos from 12 days of incubation until hatching. Fertile eggs were incubated and randomly assigned in a 2 x 6 factorial arrangement (two breeder ages - 38 and 60 weeks and six incubation days - 12, 14, 16, 18, 20, and 21). Enzymatic activity of acid and alkaline phosphatases in tibial epiphyses and weights as well as length and width in tibias and femurs of the embryos were determined. Tartrate-resistant acid and alkaline phosphatases activity in epiphyses was not affected by breeder age. Absolute weight and width of femur and tibia were larger in 60-week-old embryos compared to 38-week-old. Enzymatic activity and morphometric measurements increased with incubation day,... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Chicken embryo; Alkaline phosphatase; Epiphyses; Growth plate; Long bones. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100031 |
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| Álvarez,Marco; Perdomo,Lourdes. |
| Carbonated water is a fundamental part of many drinks and its effects have been studied in many pathological situations. However, cells and tissue damage as a consequence of carbonated water has not been the subject of extensive research. We assessed the short-term effects of soda on in vitro Hanging-drop culture of myoblasts and ex vivo lower limb of 8-day-old chicken embryo skeletal muscle tissue. Several groupswerendesigned: a) Control (Con-tyr), b) Carbonated water (Car), c) Coffe (Caf), and d) Cola beverage (Glu). The samples were observed with light microscopy and digital imaging analysis was performed. The ultra-structure of control and treated tissue were observed with electron microscopy. Immunohistochemistry techniques, such as terminal... |
| Tipo: Journal article |
Palavras-chave: Soda Club; Chicken embryo; Low limb; Apoptosis; Necrosis. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200006 |
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